A Textbook on Chromatography: An Overview

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Authors

Rajesh Gour
School of Pharmacy, LNCT University, Bhopal Madhya Pradesh, India
Pradeep Kumar Mohanty
Department of Pharmacology, School of Pharmacy, LNCT university, Bhopal, India
Vishal Shrivastava
School of Pharmacy, LNCT University, Bhopal, India
https://orcid.org/0009-0007-5184-5459
Mahadev Kanere
Department of Pharmaceutics, School of Pharmacy, LNCT University Bhopal, India

Keywords:

Chromatographic Techniques, High-Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), Gas Chromatography, Ion Exchange Chromatography, Analytical Chemistry

Synopsis

Chromatography is a process mainly used for separating the various components of a combination. The mobile phase, a liquid solution that covers the combination's components and holds them together while they travel throughout a structure containing a distinct substance which is called as the stationary phase, is what gives the mixture its name. Separation of
components requires differential partitioning between the mobile and stationary phases. The initial aim of chromatography is to separate and extract one or more components from a sample with the analytical goal of determining the sample's chemical composition, both qualitatively and quantitatively. If the fixed and movable phases are appropriately chosen, the different components can be efficiently separated. The various parts will move at various rates. For instance, when a polar solvent is utilised as the mobile phase, polar molecules are more likely to move quickly. One of the best tools analytical chemists have for assessing various analytes in complicated mixtures is chromatography. The power of chromatography resides in its ability to identify the identities (chemical structures) and amounts of various substances, or "analytes," and to discriminate between them.

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Published

20 May 2025
Copyright (c) 2025 Rajesh Gour, Pradeep Kumar Mohanty, Vishal Shrivastava, Mahadev Kanere

Details about the available publication format: E-Book

E-Book

ISBN-13 (15)

978-93-49910-60-7

Details about the available publication format: Book (Paperback)

Book (Paperback)

ISBN-13 (15)

978-93-49910-12-6

How to Cite

Gour, R. ., Mohanty, P. K. ., Shrivastava, V. ., & Kanere, M. . (2025). A Textbook on Chromatography: An Overview. Deep Science Publishing. https://doi.org/10.70593/978-93-49910-60-7
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